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Microorganisms can affect product quality, consistency, process performance, and shelf life before a problem becomes visible.
Biotrax provides third-party food microbiology testing for raw materials, ingredients, in-process samples, and finished food and beverage products. Testing can help establish microbial baselines, evaluate specifications, compare batches or suppliers, investigate quality concerns, and monitor changes over time.
Not sure which microbial analysis to request? Tell us what you are testing and what you need to learn. Our team can help clarify the available options, sample requirements, and an appropriate place to begin.
Food microbiological testing may be useful when your team needs to:
The appropriate test depends on the product, production stage, specification, and question your team is trying to answer.
These services answer different questions.
Quantitative testing estimates how many microorganisms are present in the tested sample. These analyses are often used as indicators of: product quality, process hygiene, sanitation performance, spoilage potential, shelf-life stability, and batch or supplier consistency.
Results may be reported as a Most Probable Number, or MPN, or as colony-forming units, or CFU, depending on the method used.
Pathogen testing is designed to determine whether a specific harmful organism—such as Salmonella, Listeria monocytogenes, or pathogenic E. coli—was detected in the tested portion.
A quality-indicator result does not confirm that a product is free from foodborne pathogens. When a specific pathogen is the concern, pathogen-specific testing should be requested.
Many of Biotrax’s quantitative microbiological analyses are performed using the bioMérieux TEMPO system.
TEMPO is an automated microbial enumeration platform based on the traditional Most Probable Number approach. Prepared samples are combined with test-specific media, loaded into sealed testing cards, incubated, and read automatically to estimate the concentration of viable microorganisms present.
This automated approach supports standardized processing, consistent calculations, and laboratory traceability for routine food-quality and hygiene-indicator testing.
Traditional cultural and plate-count methods may also be used when required by the product, customer specification, reference method, or testing objective.
What does "enumeration" mean?
Enumeration is a quantitative test that estimates how many microorganisms are present. It differs from a presence-or-absence test, which reports whether a specific target was detected in the tested portion.

Estimates the overall level of viable aerobic microorganisms in a sample.
Why test for it:
Aerobic count is commonly used to evaluate raw-material quality, product consistency, sanitation, processing conditions, and microbial changes during shelf life.
Important:
It does not identify individual organisms or confirm that a product is free from pathogens.
Estimates the amount of viable yeast and mold present.
Why test for it:
Yeast and mold can contribute to visible growth, fermentation, off-flavors, gas production, package swelling, and shortened product life. Testing may support spoilage investigations, ingredient evaluation, and shelf-life studies.
Important:
It does not identify individual organisms or confirm that a product is free from pathogens.
Targets fungal organisms or resistant structures capable of surviving heat exposure.
Why test for it:
This analysis may be appropriate for heat-processed or shelf-stable products where resistant spoilage organisms could survive processing and create problems later.
Important:
It does not identify individual organisms or confirm that a product is free from pathogens.
Measures coliform organisms commonly used as general indicators of sanitation and process control.
Why test for it:
Coliform testing may help evaluate raw-material conditions, cleaning effectiveness, process hygiene, or possible contamination after processing.
Important:
A coliform result does not automatically indicate fecal contamination.
Measures non-pathogen-specific Escherichia coli.
Why test for it:
Generic E. coli may be used as an indicator of fecal contamination, raw-material quality, handling practices, or process hygiene.
Important:
This is different from pathogen testing for E. coli O157:H7 or Shiga toxin-producing E. coli, or STEC.
Measures a broad family of bacteria that includes many organisms used as hygiene and process indicators.
Why test for it:
Testing may help evaluate sanitation performance, heat-process effectiveness, raw-material quality, or possible post-process contamination.
Important:
It does not identify individual organisms or confirm that a product is free from pathogens.
Measures Lactobacillus and related lactic acid bacteria.
Why test for it:
These organisms may be desirable during controlled fermentation but can also contribute to unintended souring, gas production, flavor changes, or spoilage.
Measures S. aureus associated with handling and hygiene concerns.
Why test for it:
Testing may support evaluation of employee handling, sanitation, process control, or an unexpected product result.
Important:
An S. aureus count is not the same as testing for staphylococcal enterotoxin.
Measures Bacillus cereus, a spore-forming bacterium that may survive conditions that destroy many other bacteria.
Why test for it:
It may be relevant to grains, starch-based products, dairy products, sauces, seasonings, and prepared foods where spoilage or food-safety concerns exist.
Measures Campylobacter organisms, which are most often associated with poultry and certain raw animal products.
Why test for it:
Quantitative testing may support specialized process-verification, carcass, poultry, or risk-assessment programs.
Clients should confirm whether their program requires enumeration or a different pathogen-testing approach.
Measures generic E. coli recovered from a defined carcass surface area.
Why test for it:
Carcass-swab testing may support verification of slaughter, dressing, handling hygiene, and process performance over time.

Biotrax also offers applicable traditional cultural and plating methods.
Plate-count methods grow microorganisms on agar so colonies can be counted or isolated.
They may be appropriate when:
Available options may include aerobic plate count, yeast and mold plate count, coliform and generic E. coli counts, Staphylococcus aureus count, and anaerobic plate count.
If you have a specific microogranism you're looking for that needs plating reach out to our team for additional infromation.
A microbial count is not automatically “good” or “bad” based on the number alone.
The meaning of a result may depend on:
A result represents the tested portion of one sample at one point in time. Routine testing and trend review can provide a more useful picture of product and process consistency than a single isolated result.
When contacting Biotrax, be prepared to tell us:
Food microbiology testing commonly requires at least 50 grams per sample, although more sample may be needed depending on the analyses requested and analytical portions. Contact the laboratory before collection or shipment when sample quantity, packaging, temperature, or timing is uncertain.
You do not need to select a method or test code before contacting Biotrax.
Tell us about your product, process, specification, or concern. Our team can help clarify the available food microbiology testing options, confirm sample requirements, and determine an appropriate testing path.
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